人外周血淋巴細胞

CRL-8083 MC/CAR 人外周血淋巴細胞

ATCC® Number: CRL-8083™ Price: $417.00

Designations: MC/CAR

Depositors: Research Corporation

Isotype: IgG1 （cytoplasmic）; kappa light chain

Biosafety Leee Propagation

Growth Properties: suspension

Organism: Homo sapiens （human）

Morphology: lymphoblast

CRL-8083 MC/CAR 人外周血淋巴細胞vel: 2

Shipped: frozen

Medium & Serum: S

Source: Organ: peripheral blood

Disease: plasmacytoma; myeloma

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: complement （C3b）

Tumorigenic: Yes

Antigen Expression: HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +

DNA Profile （STR）: Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11,13

D16S539: 11,12

D5S818: 11,14

D7S820: 9,13

THO1: 6,9

TPOX: 8

vWA: 17,18

Cytogenetic Analysis: normal male diploid

Isoenzymes: ADA, 1

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1

PGM3, 1

Age: 81 years

Gender: male

Ethnicity: Caucasian

Comments: Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270]

The cells produce, but do not secrete, immunoglobulin.

No surface immunoglobulin.

8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.

Can be used as a human fusion line.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%

Temperature: 37.0°C

Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984

43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762

58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719

CRL-8083 MC/CAR