人外周血淋巴細胞

CCL-120 CCRF-SD 人外周血淋巴細胞

ATCC® Number: CCL-120™ Price: $338.00

Designations: CCRF-SB [CCRF SB]

Depositors: GE Foley

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped

: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens （human

）

Morphology: lymphoblast

CCL-120 CCRF-SD 人外周血淋巴細胞

Source: Organ: peripheral blood

Disease: acute lymphoblastic leukemia

Cell Type: B lymphoblast;

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Antigen Expression: HLA A1, A2, B12, B17, Cw2

DNA Profile （STR）: Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 10,12

D16S539: 9,13

D5S818: 11,12

D7S820: 11,12

THO1: 9,10

TPOX: 8

vWA: 18

Isoenzymes: G6PD, B

Age: 11.5 years

Gender: male

Ethnicity: Caucasian

Comments: A T cell line （HSB-2, ATCC CCL-120.1） was also obtained from the same buffy coat preparation.

Derived from a buffy coat preparation from a patient with ALL.

The cells are EBNA positive.

Propagation: ATCC complete growth medium: RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 10（5） viable cells/ml.

Medium Renewal: Add fresh medium （10% to 20% by volume） every 2 to 3 days

Related Products: derived from same individual:ATCC CCL-120.1

References: 24413: . M.D. Anderson Hospital and Tumor Inst. Monograph. 21: ; 1967.

25967: Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381

CCL-120 CCRF-SD