Biosafety Level: 2 [Cells contain human papilloma virus (HPV-18) ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent (The line can be readily adapted to growth in suspension)
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: cervix
Disease: adenocarcinoma
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility: Human adenovirus 7
Vesicular stomatitis virus
Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes: G6PD, A
Age: 31 years
Gender: female
Ethnicity: Black
HeLa Markers: Y
Comments: Relatively resistant to polioviruses.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
