Designations: HEK-293 Depositors: FL Graham Biosafety Level:2 [
Growth Properties:adherentCells contain adenovirus ] Shipped: frozen Medium & Serum: See Propagation Organism:Homo sapiens Morphology:epithelial Source:Organ: embryonic kidney Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Restrictions:These cells are distributed for research purposes only. 293 cells, their products, or their derivatives may not be distributed to third parties. Applications:efficacy testing transfection host viruscide testing Receptors:vitronectin, expressed Tumorigenic:YES DNA Profile (STR): Amelogenin: X CSF1PO: 11,12 D13S317: 12,14 D16S539: 9,13 D5S818: 8,9 D7S820: 11,12 THO1: 7,9.3 TPOX: 11 vWA: 16,19 Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells. Age: fetus Comments: Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome [RF32764], it is now clear that only left end sequences are present. The line is excellent for titrating human adenoviruses. The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit. The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosomeum supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003 Derivative: ATCC CRL-10852 Derivative: ATCC CRL-12006 Derivative: ATCC CRL-12007 Derivative: ATCC CRL-12013 Derivative: ATCC CRL-12479 Derivative: ATCC CRL-2029 Derivative: ATCC CRL-2368 Purified DNA: ATCC CRL-1573D References: CRL-1573 21624: Xie QW, et al. Complementation analysis of mutants of nitric oxide synthase reveals that the active site requires two hemes. Proc. Natl. Acad. Sci. USA 93: 4891-4896, 1996. PubMed: 8643499 21631: Da Costa LT, et al. Converting cancer genes into killer genes. Proc. Natl. Acad. Sci. USA 93: 4192-4196, 1996. PubMed: 8633039 22282: Graham FL, et al. 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