CHO-K1 (ATCC® CCL-61™) CHO-K1 倉鼠卵巢細胞亞株 Organism Cricetulus griseus, hamster, Chinese Tissue ovary Product Format frozen Morphology epithelial-like Culture Properties adherent Biosafety Level 1 Gender female Applications This cell line is suitable as a transfection host. Storage Conditions liquid nitrogen vapor phase Karyotype Chromosome Frequency Distribution 50 Cells: 2n = 22. Stemline number is hypodiploid. Derivation The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957. Clinical Data female Virus Susceptibility Vesicular stomatitis, Orsay (Indiana) Vesicular stomatitis, Glasgow (Indiana) Getah virus Virus Resistance poliovirus 2; modoc virus; Button Willow virus Comments CHO-K1 倉鼠卵巢細胞亞株 The cells require proline in the medium for growth. Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended Medium Renewal: Once or twice between subculture Cryopreservation CHO-K1 倉鼠卵巢細胞亞株 Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37°C
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) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
